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Korean J Gastroenterol  <  Volume 74(4); 2019 <  Articles

Korean J Gastroenterol 2019; 74(4): 219-226  https://doi.org/10.4166/kjg.2019.74.4.219
Screening of Sera from Patients with Pancreatitis by an Apoptosis Assay of Skin-derived Cells
Ae Eun Seok1, Byoung Kwan Son2, Jiyeong Lee3, Kwang Hyun Chung2, You-Rim Lee1, Doojin Kim4, Byung Heun Cha3 and Hee-Gyoo Kang1,3
Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University1, Daejeon; Division of Gastroenterology, Department of Internal Medicine, Eulji University Eulji Hospital2, Seoul; Department of Biomedical Laboratory Science, College of Health Sciences, Eulji University3, Seongnam; Department of Laboratory Medicine, Seongnam Central Hospital4, Seongnam, Korea
Correspondence to: Hee-Gyoo Kang, Department of Biomedical Laboratory Science, College of Health Sciences, Eulji University, 553 Sanseong-daero, Sujeong-gu, Seongnam 13135, Korea. Tel: +82-31-740-7315, Fax: +82-31-740-7315, Email: kanghg@eulji.ac.kr, ORCID: https://orcid.org/0000-0001-8690-2483
Received: April 18, 2019; Revised: August 1, 2019; Accepted: August 14, 2019; Published online: October 25, 2019.
© The Korean Journal of Gastroenterology. All rights reserved.

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background/Aims: An excessive inflammatory response is typical in acute pancreatitis and a significant cause of early mortality in severe acute pancreatitis. This is believed to be caused by inflammatory molecules or upregulated cytokine levels in the serum of patients. The aim of this study was to identify the serum-mediated apoptosis-inducing effects in acute pancreatitis patients.
Methods: A skin tissue-derived cell line, BJ, was treated for 24 hours with the sera of 22 healthy volunteers (control) and 71 acute pancreatitis patients (22 with gallstone pancreatitis, 16 with alcoholic pancreatitis, and 11 with pancreatitis with other causes) collected at the time of hospital admission (active) and discharge (resolved). Apoptosis was analyzed by flow cytometry.
Results: The average percentage of living cells, early apoptotic cells, and late apoptotic cells ranged from 78.8% to 85.0%, 5.5% to 7.3%, and 7.7% to 13.1%, respectively. The number of live cells increased significantly using the serum from the resolved state of gallstone-induced pancreatitis. In addition, the number of early apoptotic cells increased significantly using the serum from the resolved state of pancreatitis with other causes. The number of late apoptotic cells decreased significantly with the serum from the resolved state compared to the active state of gallstone- and alcohol-induced pancreatitis.
Conclusions: Serum samples from patients with pancreatitis induced a change in the apoptosis profiles of skin-derived cells. These results indicate changes in the serum components in patients with acute pancreatitis.
Keywords: Acute pancreatitis; Skin tissue-derived cell line; Apoptosis; Alcohol-induced pancreatitis


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